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Epithelial Cell Biology Core

Services provided by the Core are subsidized and on a charge-back basis. They are designed to achieve economies of scale for purchase of supplies and labor so that the real costs of epithethial cell culture are subsidized by approximately 50% for individual investigators.


Services Provided HDDC Member Fee ($) Non-member Fee ($)
Polarized Epithelial Culture (24-well format)
35
70
Polarized Epithelial Culture (6-well format)
32
64
Polarized Epithelial Culture (45cm2)
35
70
Cell line (frozen vial or flask)
50
100
Non-polarized culture (96 to 6 well formats)
5
10
Adenovirus, lentivirus Preparations
2-3000 (TBA)
N/A
Purchase cell culture supplies/media only
At cost
N/A

Specialized cells and reagents available upon request

  • Polarized Epithelial Cells
  • cDNAs and Recombinant Proteins
  • Novel small organic compounds
  • Well-characterized antibodies
  • Xenopus oocytes

Core Instrumentation and Equipment Available for Independent Use

Access is provided to Center members for no charge. The Center subsidizes the full cost of training, maintenance, supervision, and consultation for studies using these services. These core resources are provided under the direction of Drs. Lencer, Colgan, and Alper, some in collaboration with the imaging Core B, and only after appropriate training.

Specialized instrumentation located in the Core Laboratory at CH (Dr. Lencer):

1) Instrumentation for transepithelial electrophysiology and flux studies (Ussing chambers and voltage clamp modified for tissue and cell culture.)

2) Instrumentation for measurement of intracellular Ca2+ and pH transients (or at steady state) in live cells by microflourimetry (see description in Imaging Core).

3) FPLC (Bio-Rad) for recombinant or native protein purification.

4) Specialized incubators, microscopes, and aquariums for pilot studies using zebrafish as a tractable vertebrate model for study of GI development and function.

Specialized instrumentation located in the Core Laboratory at BWH (Dr. Colgan):

1) Flourescence and UV spectroscopy

2) Biacore analysis

3) Hypoxia chamber

Specialized instrumentation located in the Core Laboratory at BIDMC (Dr. Alper):

1) AMAXA nucleofection device - high efficiency electroporation for primary epithelial cells.

2) Nanodrop spectrophotometer that uses 1 microliter sample size without need for cuvette

3) Xenopus oocyte microinjector and dissecting scape, micropipette puller

4) ECIS (Electric Cell-substrate impedance sensing) device (Applied Biophysics)